Dyes. Your students will gain experience with the principles and practice of gel electrophoresis without the extra time and expense of running DNA. This product consists of prepared solutions of 4 dyes in water/glycerol.
Xylene cyanol
Orange G
Bromophenol blue
Methyl green
Due to differing polarity, load these dyes into the central well of the gel. The first 3 are negatively charged and migrate to the positive electrode, but Methyl Green carries a positive charge and migrates to the negative electrode. Glycerol in the solvent increases the density of the solutions so they sink into the wells and behave more like micro-samples of DNA mixed with buffered loading dye.
Since the dyes are visible in the gel, your students wull observe the entire separation process a sit unfolds, and no extra staining step is required.
Volume supplied = approx 450ul of each dye solution (0.2%w/v) plus two vials containing different dye mixtures ( approx 450ul each).