Dyes for electrophoresis

Dyes. Your students will gain experience with the principles and practice of gel electrophoresis without the extra time and expense of running DNA. This product consists of prepared solutions of 4 dyes in water/glycerol.

Xylene cyanol

Orange G

Bromophenol blue

Methyl green

Due to differing polarity, load these dyes into the central well of the gel. The first 3 are negatively charged and migrate to the positive electrode, but Methyl Green carries a positive charge and migrates to  the negative electrode. Glycerol in the solvent increases the density of the solutions so they sink into the wells and behave more like micro-samples of DNA mixed with buffered loading dye.

Since the dyes are visible in the gel, your students wull observe the entire separation process a sit unfolds, and no extra staining step is required.

Volume supplied = approx 450ul of each dye solution (0.2%w/v) plus two vials containing different dye mixtures ( approx 450ul each).